Vol. 39, No. 1-2, 1995
DNA repair in peripheral blood lymphocytes from cattle infected with bovine leukemia virus
Stec, J., Grundboeck, J., Reichert, M.
National Veterinary Research Institute
Department of Biochemistry
24-100 Pulawy
Poland
Summary
DNA repair synthesis was estimated in bovine peripheral blood lymphocytes in vitro by measurement of the hydroxyurea insensitive incorporation of 3H-thymidine into DNA. The cells were treated with UV light or benzo (alfa) pyrene-dial-epoxide (BPDE). The current study was performed with lymphocytes from healthy and BLV (bovine leukemia virus) infected cattle. All leukemic animals had specific antibodies in the serum detected by AGID and ELISA.
UV-induced repair in lymphocytes from BLV infected cattle was very similar to that of healthy animals, and the difference was not statistically significant (p > 0.05). When lymphocytes were treated with BPDE, the replicative DNA synthesis and the DNA excision repair decreased according to the applied dosage. Lymphocytes from BLV infected cattle showed a lower ability to repair DNA damages made by BPDE than normal animals. The greatest differences were revealed within a range of PBDE concentration from 0.6 to 19.2 mig/ml, and were statistically significant (p < 0.05).
Key words: DNA repair; bovine lymphocytes; bovine leukemia virus; benzol (alfa) pirene; UV light
References
Folia Veterinaria / 1995