Vol. 39, No. 3-4, 1995
The effects of postischaemic hyperoxia of the granular cells of the bulbus olfactorius in dogs
Zachariáš, L., Mechírová, E*., Maršala, M*., Vanický, I*., Maršala, J.
The Faculty of Medicine
The University of P. J. Safarik
Srobarova 2
040 01 Kosice |
* Institute of Neurobiology of the SAC
Soltesovej 6
040 01 Kosice
Slovak Republic |
Summary
The effects of acute postischaemic reoxygenation on the nervous cells of the bulbus olfactorius were studied. The aim of this study was to investigate whether acute changes of neurones arise during ischaemia or during hyperoxic recirculation.
In experimental animals, potassium cardiac arrest was induced for 15 min. After restoration of heart and breathing activities, one group of five dogs was left to inspirate hyperbaric oxygen (pO2 = 460 mm Hg) for 1 hour of recirculation. After one hour of recirculation the bulbus olfactorius was removed, fixed, and processed by Nauta's impregnation method (N a u t a, 1957). In the second group of three dogs, after 15 min of ischaemia, samples for histological examination were taken immediately without restoring the of circulation.
In a dogs with hyperoxic recirculation, examination of the bulbus olfactorius revealed groups of almost black impregnated granular cells, which had succumbed to acute neuronal death. In the dogs without postischaemic restoration of circulation, such changes of neurones in granular layer of the bulbus olfactorius were missing.
These results indicate that acute neuronal death probably occurs due to oxygen burst during recirculation.
Key words: cardiac arrest; hyperbaric reoxygenation; bulbus olfactorius; dog; Nauta's method
This article is dedicated to the memory of the late Prof. P. P o p e s k o, DVM, DSc on the seventieth anniversary of his birth.
References
Folia Veterinaria / 1995