Vol. 41, No. 3-4, 1997 content next

A QUANTITATIVE METHOD FOR DETERMINING THE IMMUNOGENIC ACTIVITY OF ORAL ANTIRABIES VACCINES

Švrček, S., Ondrejka, R., Beníšek, Z., Závadová J.*, Ďurove, A., Süliová, J., Maďar, M.*

University of Veterinary Medicine
Komenskeho 73
041 81 Kosice
Slovak Republic		 * Research Institute of Experimental Veterinary Medicine
Hlinkova 1/A
040 00 Košice
The Slovak Republic

Summary

A method for the quantitative determination of the immunogenic activity of oral antirabies vaccines in model experiments on mice is described. The procedure imitates the vaccination of the target animal species and takes into consideration the specificities of oral antirabies vaccination. The method is strictly quantitative and easily reproducible. It presents a purposeful oral way of applying of a known quantity of vaccine and a natural - peripheral (subcutaneous) - way of challenge. The immunogenic activity of the oral vaccine is expressed quantitatively as ED50 of the vaccine against the subsequent challenge defined by the quantity of 50 % mouse subcutaneous dosis lethalis (MSCLD50) administered. The ED50 value is expressed as the vaccine dilution required or the quantity of an undiluted suspension of the vaccination strain in pl (infectious tissue culture medium). A formula for calculating the effectiveness of a vaccine in the form of ED50 together with a worked example are given.

Key words: rabies; immunoprophylaxis of rabies; oral antirabies immunization; method for testing the immunogenic activity of an oral antirabies vaccin

References

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Folia Veterinaria / 1997