Vol. 41, No. 3-4, 1997
USAGE OF RECOMBINANT RECEPTOR PROTEINS IN CLINICAL DIAGNOSIS
Gupalova, T., Orlova, S., Palagnut, V., Volchek, N., Kuzmina, M., Totolian, A.
Institute of Experimental Medicine RAMS
St.-Petersburg
Acad. Pavlova 12
197376 Russia
Summary
The fragment of gene encoding 31 kD albumin binding protein and fragment of gene encoding 31 kD IgG binding protein were cloned. The immunochemical properties of these proteins were characterized. Methods for the quantitative evaluation of proteins (albumin and IgGs) in human biological fluids (mainly in urine) were developed. Since these methods are based on the ability of recombinant receptor proteins to interact only with the respective plasma protein the reactions proved to be very specific and sensitive. Besides, these methods are simple and may be used in everyday clinical practice and also in the population for screening investigations. These methods were put through clinic trials.
Key words: streptococcus; protein G; albumin and IgG binding; microalbuminuria
References
Folia Veterinaria / 1997