Vol. 43, No. 4, 1999
THE FUNCTIONAL ACTIVITY OF BLOOD LYMPHOCYTES AND PHAGOCYTES AFTER SKIN PAPILLOMAS EXCISION IN BULL CALVES
Paulík, Š., Ledecký, V., Mojžišová, J., Janda, J., Švrček, Š.
University of Veterinary Medicine
Komenskeho 73
041 81 Kosice
The Slovak Republic
Summary
Cell-mediated immunity as a part of the immune defence mechanism was studied in 3 bulls which undergone the skin papillomas excision. Anaesthesia (combination atropin + xylasine + procaine) together with surgery (papillomas excision), caused a significant decrease of the fluorescence intensity of cultivated lymphocytes stimulated with T-cell mitogens and the phagocytizing leukocytes. More significant suppression of proliferative response was demonstrated in lymphocytes stimulated with Con A in comparison to lymphocytes stimulated with PHA. The decrease of fluorescence intensity of non-stimulated lymphocytes and ingestion capacity of phagocytes was nonsignificant. At the same time a decrease of total leukocyte count and the increase of absolute lymphocyte count were found out. Though the restoration of the activity of lymphocytes and phagocytes started on the 2nd day after surgery, their level was still significantly lower than before surgery. Experience acquired from this study indicate that anaesthesia in connection with surgery can cause a temporary inhibition of the cell-mediated immunity in bull calves.
Key words: bull calves; papillomatosis; surgery; proliferation of lymphocytes; phagocytic activity
References
Folia Veterinaria / 1999