Vol. 43, No. 4, 1999
POST-IRRADIATION CHANGES IN MYELOGRAM OF CHICKENS
Sesztáková, E., Beňová, K., kardová, I., Toropila, M., Leistein, R.
University of Veterinary Medicine
Komenskeho 73
041 81 Kosice
The Slovak Republic
Summary
In our experiment (n = 24) we evaluated the myelogram of 37 days old broiler chickens irradiated with gamma rays in comparison with the intact control group. Experimental chickens were irradiated using a single all-body dose of 4.5 Gy at an input of 0.33 Gy/min, SSD = 95 cm.
The chickens were decapitated at 1-, 6- and 24-hour intervals post-irradiation. The bone marrow was obtained from the proximal epiphysis of the femur after its previous preparation.
The inspection examination of the bone marrow and numerical evaluation of myelogram were performed in all the groups.
Weak anaemia and slightly fatty consistency of the bone marrow were determined by autopsy of the marrow of the experimental chickens. Microscopic examination pointed out qualitative changes such as karyorrhexis, karyolysis, cellular disintegration or multiplication of undifferentiated cells.
The numerical evaluation of the myelogram confirmed inhibition of erythropoiesis and myelopoiesis. Blasts and lymphocytes were the most radiosensitive. Promyelocytes, myelocytes and granulocytes showed lower radiosensitivity.
The findings in the myelogram of the experimental chickens indicate slight aplastic anaemia.
Key words: gamma radiation; chickens; haematopoiesis; myelogram
References
Folia Veterinaria / 1999