Vol. 44, No. 1, 2000 previous next

CIRCADIAN OSCILLATIONS OF ACTIVITIES OF RAT LIVER ADAPTIVE ENZYMES DURING A SHORT-TERM FEED DEPRIVATION

Toropila, M., Ahlers, I.*, Ahlersová, E.*, Ondrašovič, M., Danko, J., Falis, M., Švický, E.

University of Veterinary Medicine
Komenského 73
041 81 Košice
The Slovak Republic		 * Šafárik University, Faculty of Science
Moyzesova 11
041 76 Košice
The Slovak Republic

Summary

After a 3-week period of adaptation to a standard artificial lighting regimen: light : darkness - 12 h : 12 h (07.00-19.00 h light, 19.00-07.00 h darkness), the experimental animals, male rats of Wistar strain SPF breeding, were deprived of feed starting at 08.00 h. During the subsequent 48 h (animals were deprived of feed for 0 - 48 h), activities of selected adaptive enzymes, tyrosine aminotransferase (TAT), tryptophan-2-3-dioxygenase (TO), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were determined in the liver cytosol fraction at 3-h intervals.
Although the rhythm of circadian oscillations of selected enzyme activities was preserved in the animals fasting for different periods, some characteristics of the activities changed in comparison with well-fed animals, particularly during the dark period of the day.

Key words: circadian oscillations; adaptive enzymes; liver; rat

References

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Folia Veterinaria / 2000