Vol. 45, No. 1, 2001 content next

THE USE OF A MOLECULAR TYPING METHOD TO EVALUATE THE TESTING OF Listeria monocytogenes CONTAMINATION OF RAW MILK AND DAIRY PRODUCTS

Tkáčiková, Ľ. 1, Kantíková, M. 2, Dmitriev, A. 3, Mikula, Ľ. 4

1 University of Veterinary Medicine
Košice
Slovakia		 2 State veterinary institute
Dolný Kubín
Slovakia		 3 Institute of Experimental Medicine
St-Petersburg
Russia		 4 Neuroimmunological Institute SAS
Bratislava
Slovakia
This paper was originally presented at the 2nd Middle-European Congress for Buiatrics held in Stará Lesná. The High Tatras, from the 10th to 13th May 2000

Summary

Pulsed-field gel electrophoresis (PFGE) and DNA-DNA hybridisation with an rrs gene probe were used to compare 19 serogroup 1/2a Listeria monocytogenes strains isolated from raw milk, dairy products, and salt water in one dairy processing plant in order to determine whether the strains isolated over an 8-month period were similar, i.e. if they belonged to the same clone. Results of PFGE analysis showed that the samples of raw milk were contaminated by seven L. monocytogenes clones. The L. monocytogenes isolated from dairy products and salt water were identical and were distinct from the clones of L. monocytogenes isolated from raw milk.

Key words: cattle; Listeria monocytogenes; molecular typing; raw milk; dairy products

References

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Folia Veterinaria / 2001