Vol. 45, No. 2, 2001 content next

A GENETIC ANALYSIS OF BVDV ISOLATES ORIGINATING IN EUROPE

Vilček, Š. 1, Ďurkovič, B. 2, Strojný, L. 2, Rossmanith, W. 3, Loitsch, A. 4, Moussa, A. 5, Paton, D. J. 6

1University of Veterinary Medicine
Komenského 73
041 81 Košice
The Slovak Republic		 2Research Institute of Veterinary Medicine
Duklianskych hrdinov 1A
040 00 Košice
The Slovak Republic		 3Veterinary Office
Government of Lower Austria
Landhausplatz 1
A-3109 St. Polten
Austria		 4Federal Institute for Control of Viral Infection in Animals
Robert Kochgasse 17
A-2340 Moedling
Austria		 5Agence Francaise de Securite Sanitaire des Aliments
31 Avenue Tony Garnier, BP 7033
F-69364 Lyon Cedex 07
France		 6Veterinary Laboratories Agency (Weybridge)
Addlestone
Surrey KT15 3NB
United Kingdom
This paper was originally presented at the 2nd Middle-European Congress for Buiatrics held in Stará Lesná. The High Tatras, from the 10th to 13th May 2000

Summary

Seventy-eight bovine viral diarrhoea viruses (BVDV) recently collected in Austria, France, Hungary, Italy, Slovakia, Spain, and UK were genetically typed in the 5'- untranslated (5'UTR) and autoprotease (Npro) regions of the pestivirus genome. Seventy-six of the isolates were BVDV-1 and two French isolates were of the BVDV-2 genotype. Phylogenetic analysis of the 5'UTR (245 nt), including additional BVDV-1 sequences from USA, Canada, Germany, New Zealand, Mozambique and Sweden, taken from GenBank and from our previous works, indicated that these viruses were clustered not only into the two generally accepted groups (BVDV-1a - "NADL like" and BVDV-1b - "Osloss like"), but altogether in 11 phylogenetic groups. Similar clustering was observed with Npro region sequences (385 nt) and the highest bootstrap values (over 95 %) were obtained by phylogeny combining 5'UTR and Npro sequences.

Key words: cattle; BVD isolates; genetic analysis; Europe

References

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Folia Veterinaria / 2001