Vol. 45, No. 4, 2001
LOCALIZATION OF ACETYLATED TUBULIN-POSITIVE NERVE FIBRES IN THE SPLEEN OF SHEEP
Marettová, E., Maretta, M.
University of Veterinary Mediciny
Komenského 73
041 81 Košice
The Slovak Republic
Summary
The distribution of acetylated tubulin in the sheep spleen was studied using an indirect immunohistochemical method. A strong positive reaction for acetylated tubulin was observed on the nerve trunks and fine nerve fibres localized in the spleen hilus, and fine nerve fibres were seen in the fibrous capsule and trabeculae. Accompanying large and medium-sized branches of the splenic artery, nerve fibres enter the splenic parenchyma and as such are distributed in the white pulp. Most of the spleen nerve fibres positive for acetylated tubulin were found in the perivascular area, particularly arteries, arterioles, and the central artery of the splenic follicle. A fine network of nerve fibres was observed in the red pulp. After using the immunohistochemical method and acetylated tubulin antibody (Sigma), the present study displays the localization of nerve fibres common in mammals.
Key words: acetylated tubulin; nerve fibres; spleen; sheep; immunohistochemistry
References
Folia Veterinaria / 2001