Vol. 45, No. 4, 2001 content next

THE ISOENZYME SPECTRUM OF SERUM LACTATE DEHYDROGENASE IN ENZOOTIC BOVINE LEUKOSIS

Buleca, J., Bíreš, J., *Šutiaková, I., Buleca, J., Jr., Švický, E., Pilipčinec, E., Bugarský, A.

University of Veterinary Medicine
Komenského 73
041 81 Košice
The Slovak Republic		 *Research Institute of Veterinary Medicine
Hlinkova 3
040 01 Košice
The Slovak Republic

Summary

In our work the results of enzymological analyses of the isoenzyme spectrum of lactate dehydrogenase in the blood serum of cattle are presented. The occurrence of enzootic bovine leukosis evoke interest of using electrophoretical analyses of lactate dehydrogenase isoenzymes as one possible marker of the manifestation of leukaemogenesis, or the onset of pre-leukosis initiation. Using a densitometrical evaluation of electrophoretograms of the blood serum of cattle, a decreasing trend in the individual fractions LDH1 to LDH5 were noticed, as well as a significantly low occurrence of the minor fractions LDH4 and LDH5. Serological examinations of ELISA tests were done in parallel. Significant subfractions of LDH1 isoenzyme in the electrophoretical analysis of EBL positive animals were observed.

Key words: cattle; enzootic bovine leukosis; lactate dehydrogenase; isoenzymes

References

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Folia Veterinaria / 2001