KOVÁČOVÁ, D., MIHALIČOVÁ, D., PAKÁNOVÁ, M.:
Precipitating antigen Brucella ovis for serological examination of ovine epididymitis.
Brucella ovis is the cause of ovine epididymitis. Its occurence is monitored by the clinical examination of genitals in rams, especially in markets. Clinical diagnosis is not sufficient, so our institute has developed a reliable serological diagnostic procedure. In the first stage, we have improved the culturing methods for isolation of B. ovis in the epididymis or in the semen samples. Several strains of B. ovis have been obtained that were used for the preparation of an antigen. In the second stage, the antigen was tested in various serological tests: CF (complement fixation) and AGID (agar gel immunodiffusion). In the third stage, the standardisation of the antigen Brucella ovis has been solved. The strain Brucella melitensis CAMP 6374 from the Collection of zoopathogenic microbes in the Research Institute of Veterinary Medicine in Brno, Czech Republic was used as a source of antigen. The Institute of Industrial Ownership in Banská Bystrica has entitled the patent number 280264 "The method for preparation of the precipitating antigen Brucella ovis" for the agar gel immunodiffusion for the staff of the State Veterinary Institute in Prešov on July 15, 1999.
Key words: infectious epididymitis in rams; Brucella ovis; complement fixating test; immunodiffusion test; precipitating antibodies
Respiratory diseases in horses, like in other animals, belong to the group of serious diseases, in which viruses, bacteria and fungi participate.
Pneumopathies of viral origin were described in our previous works (Pilipčinec et al., 1999 a, b).
Infections of the respiratory tract in horses are caused primarily by Streptococcus equi subsp. equi, Rhodococcus equi and Streptococcus equi subsp. zooepidemicus. As well, secondary bacterial bronchopneumonia and pleuropneumonia are significant.
At pneumomycosis in horses, infections induced by Histoplasma capsulatum, Coccidioides immitis and Cryptococcus neoformans have the highest significance.
Clinical diagnosis of respiratory diseases requires supplementation of laboratory examination, especially at the first distribution in the breeding (locality) and in valuable animals.
Immunization of horses against viral pathogens of respiratory diseases is one of significant factors of the control and prevention of secondary bacterial bronchopneumonia and pleuropneumonia.
Pathogenesis, clinical symptoms, patho-anatomical finding, diagnostics and prevention of serious respiratory diseases in horses induced by bacteria and fungi are described.
Key words: respiratory diseases in horses; bronchopneumonia; Rhodococcus equi
The purpose of the presented study was to follow the relation between the prophylactic and therapeutic autogenous vaccination against bovine papillomatosis with two kind of vaccines, prepared from the wart tissues (nonpurified vaccine - A and semipurified vaccine - B) and some blood constituents of metabolic profiles. Each animal was twice intramuscularly injected (10 day interval) with 5 ml vaccine A (11.50 mg proteins.ml-1) or vaccine B (3.17 mg proteins.ml-1). The treatment with vaccine showed 93.5 % efficiency without difference of the used vaccine after 105 days of vaccination. Papillomavirus infection of animals was connected with serious disorders of the metabolism (mainly mineral, energetic and nitrous) probably caused by damage of the liver and kidney with mutagenic, carcinogenic and immunosuppressive cadmium, arsenic and lead, which we observed in the serum of tested animals.
Key words: bovine papillomatosis; autovaccination; nonpurified vaccine, semipurified vaccine; tumour glycoproteins; metabolic profiles; heavy metals
Invasiveness with bots in Slovak Republic in hoofed game is high; while in roe deer it reaches 53 to 65 %, in red deer and fallow deer it is from 41 to 49 %.
The invasiveness trend is different in various animal species. While in roe deer the percentage of invasiveness increases, in red deer and fallow deer decreases. Natural and ecological conditions may be considered as a potential cause of different invasiveness; the northern districts had lower invasiveness than southern ones. Both the prevention and therapy in the form of premixes manifest positively in red deer.
Key words: hoofed game; roe, red, fallow deer; nasal and dermal bot infestation
During 1997 and 1998, 849 hearts of pigs for slaughter of 6 breeds were examined at the fattening station. Of this number, 455 hearts were from pigs of White improved, 103 of Landrasse, 124 of Pietraine, 32 of Duroc, 61 of Slovak meat and 74 of Yorkshire. Based upon the fattening parameters, the mean food consumption per 1 kg of weight gain and mean daily gain were observed in individual breeds.
Foramen ovale patens occurred in 68 hearts of White improved (15 %), 14 hearts of Landrasse (13.4 %), in two hearts of Slovak meat and in two of Yorkshire breed. In the group of Duroc and Pietraine, foramen ovale patens was not observed. After comparison to the food consumption, the highest consumption was recorded in the groups of White improved and Landrasse, and the lowest one in the groups of Yorkshire, Slovak meat, Pietraine. Contrariwise, the highest weight gain were recorded in the breed Yorkshire and Slovak meat. In the groups of White improved and Landrasse, the gain were apparently lower. Thus, it may be concluded that there is a certain real dependence between the occurrence of foramen ovale patens, food consumption and its utilization as well as weight gain in pig fattening.
Key words: fattening; foramen ovale patens; food consumption; weight gain
In the work, the influence of a synthetic sound stimulation on the hatching Hampshire chicken embryo was studied. For stimulation an electronic sound generator with amplitude of power 1250 mV and the time interval of 176 ms and 380 ms was used. After using the sound stimulation on chicken embryo, the best results were achieved in the experimental sound stimulated groups (earlier beginning of beakclapping, shorter time of breakclapping in the whole group and hatching time), in comparison with control groups, where the set eggs were hatched without sound stimulation.
Key words: synthetic sound; hatching; chicken
The elevated occurrence of abnormal forms of behaviour in dogs indicates to a disproportion between expansion of their breeding and a man failure in this process. Both the professional and social orders actualize veterinary ethology in the veterinary medicine and practice. Diagnostics, therapy of ethopathies as well as their frequent forms are discussed here.
Key words: dog; clinical ethology; abnormal behaviour
In three experiments on horse, sheep and pigs naturally infected with gastrointestinal nematodes, pyrantel tartrate in the anthelmintic drug PIRITEL plv. a. u. v. was orally applied in the recommended doses.
In the experiment on horses, the drug was effective against Parascaris equorum (97.8 - 100%) and Strongylus spp. (97.9 - 100%) in 28 days interval.
In the experiment on sheep, pyrantel tartrate showed 99.1% efficacy against gastrointestinal nematodes. By cultivation to L3 the following species composition was found: Chabertia ovina, Ostertagia spp., Bunostomun trigocephalum, Haemonchus contortus, Trichonstrongylus spp., Oesophagostomum spp., and Strongyloides papillosus.
In the experiment on pigs during 14 days the drug had 98.7% efficacy against Ascaris suum and 100% against Oesophagostomum spp., while against Trichuris suis the efficacy was only 88.6%.
The treated animals accepted the medicated food well and no side effects were observed.
Key words: gastrointestinal nematodes; pyrantel tartrate; horses; sheep; pigs
In this study, the presence and distribution of NADPH-diaphorase structures in the pheasant thoracic spinal cord are demonstrated. Histochemical method was used for visualization of NADPH-d activity. Three important regions with diaphorase positivity were found in the spinal cord, the superficial laminae of the dorsal horn, pericentral region and deep laminae of the ventral horn. The presence of NADPH-d structures in these parts suggests the participation of NO in the spinal sensory processes and therefore also visceral functions in the spinal cord of pheasant. Exact determination of NO role will need further investigations.
Key words: NADPH-d; NO; spinal cord; pheasant
It is possible to explain logically an increase in the number of disputed cases, where the essence is compensation of the property damage - an animal of the injured breeder - by the change of ownership relations. Therefore, it is necessary to increase attention to the study of the problems concerning forensic veterinary medicine in Slovak Republic. This contribution points out the basic principles which have to be respected at the official veterinary autopsy and at the inspection of a dead body on the place of finding as well as in a written report (expert opinion).
Key words: official veterinary autopsy; inspection of dead body; postmortal changes; documentation